What is Bacillus Subttilis (Microbes)?


Bacillus subtilis, also known as grass bacillus or hay bacillus, is a rod-shaped bacterium. It is found in soul and the gastrointestional tract of ruminants and humans. As with other members of the genus Bacillus, it can form and endospore to survive extreme environmental conditions of temperature and desiccation. It is one of the bacterial champions in secreted enzyme production and use on an industrial scale by biotechnology companies. Magnification is 10,000x at 15cm image size. ClearBlu offers the highest strength industrial grade bacteria available in two blends. Bred and blended in sterile lab conditions, these bacteria will dramactically improve the digestion process in your treatment plant.

ClearBlu Dry Microbes

ClearBlu dry microbes are available in blends with all bacillus strains, or with bacillus and two strains of pseudomonas and come freeze-dried on wheat bran or a salt/sugar carrier. Bacillus only blends are effective for municipal or industrial waste streams with minimal oil and grease. For waste streams with oil and/or grease applications (non-synthetic), blends with pseudomonas are preferred. In addition, pseudomona putida has been shown to act as an aerobic denitrifier. Using this blend for your treatment can reduce the load on your denitrification plant, or eliminate it completely. Bacillus blends are available in 1, 5 and 10 billion colony forming units per gram; or 1×108 CFU's, 1×109 CFU's, and 1×1010 CFU's. Blends with pseudomonas are available in 1 and 5 billion CFU's only; or 1×108 CFU's and 1×109 CFU's.

Almost all commercially available bacteria blends only contain a mix of Bacillus. Bacillus is an excellent treatment of bacteria in wastewater but is best suited for treating fats, oils, greases, and proteins. That is why they are primarily used in wastewater treatment plants. The primary constituents of brewery, winery, and food processor waste are sugars and carbohydrates in very high concentrations. This makes their waste dramatically different than sewage treatment plants. The best bacteria for breaking down sugars and carbs are Pseudomonas. Pseudomonas will act very quickly on them and will reduce BOD levels far more rapidly. The blend ClearBlu offers contains multiple strains of Bacillus as well as two proven strains of Pseudomonas: Pseudomonas fluorescens and Pseudomonas putida. Both have additional important benefits. Fluorescens will grow in water temperatures as low as 5 degrees C which makes it far more beneficial than Bacillus in treatment ponds during winter months. Putida has the added benefit of converting nitrates to nitrites. For all new and renewed discharge permits many state agencies are now requiring limits for nitrates as they have become a significant contributor to groundwater contamination. Putida, in addition to its work on carbohydrates, will aerobically convert nitrates to nitrites which can be readily consumed by plants.

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ClearBlu Liquid Microbes

ClearBlu liquid microbes are available in bacillus only blends and are excellent for municipal plants and most food processing applications. They do an excellent job of digesting organic loads, lowering BOD and digesting sludge. They are available in 1, 5 and 10 billion colony forming units per oz., or per 30 ml; or 1×108 CFU's, 1×109 CFU's, and 1×1010 CFU's per oz., or per 30 ml.

  1. I have a consistent strength and flow. If your flow is relatively low with minimal oversight, a 1×108 CFU blend is a good choice because the cost is lower and the dosing is flexible. You can dose liquid bacillus only with a peristaltic pump, dry packets, or bulk dry pails.
  2. I have high strength (over 4,000 mg/L BOD) and a high flow (over 100,000 gallons per day, or 380 M \. Using dry bacteria will save significant dollars in terms of shipping costs. If you can adequately control dosing with staff, or with the use of a volumetric doser, the highest strength bacteria is the most cost-effective solution. This option also allows you to use a pseudomona blend if you have oil and grease, or need to aerobically denitrify.

Use our bacteria calculator to determine the amount to dose. These are general guidelines. Dosing rates will be affected by a number of factors. For instance, the ideal bacteria/nutrient ratio is 100:5:1, or 100 parts BOD to 5 parts nitrogen and 1 part phosphorus. If you waste stream is low on nutrients, more retention time can achieve the desired results. With proper aeration and the addition of bacteria, a 98% plus reduction in BOD is possible. It is also possible to digest organic sludge instead of separating it for disposal.

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Results of Routine ClearBlu Microbe Use


What is Bio-Block?

Bio-Block is a slow dissolve, bacterial-laden solid for use in degrading organic waste in higher flow systems such as lift stations, wet wells, etc. The safe, naturally- occurring bacteria are present in high numbers to handle difficult organic problems.

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What is Bio-Brick?

Bio-BrickTM is a slow dissolve, bacterial-laden solid for use in degrading organic waste in grease traps. The safe, naturally-occurring bacteria are present in high numbers to handle difficult organic problems. The unique brick will gradually dissolve over a 35 – 120 day period, which allows for continuous treatment and degradation of waste. The naturally-occurring bacteria contained in the brick will reduce odor, sludge, fats, oils and grease buildup.

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Case Studies


organic microbes

Castoro Cellars

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winery microbes

Conn Creek Winery

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vineyard microbes

Daou Winery

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winery microbes

Jordan Winery

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microbes

Scheid Vineyards

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microbes

Jackson Family Wines

brewey microbes

Anderson Valley Brewing Company

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Additional Resources